SEMINAR WITH A/PROF SHAUN LOTT

SEMINAR WITH A/PROF SHAUN LOTT

Start Date: 29 May 2020 – 2:00pm
End Date: 29 May 2020 – 3:00pm
Zoom link: https://latrobe.zoom.us/j/98075898622
Meeting ID: 980 7589 8622
For additional information, please contact Prof. Brian Abbey
E: B.Abbey@latrobe.edu.au

    • Category: Events
    • Date: May 27, 2020
    • Added By: ImagingCoE

A/PROF SHAUN LOTT
The University of Auckland

“RNase HI as a target for new anti-bacterials.”

RNase HI activity is essential in all cells, as it is required to resolve R-loops, which are three-stranded nucleic acid structures that occur during transcription when the nascent RNA hybridises to the template strand of DNA. To investigate whether RNase HI would be a tractable target for new anti-bacterial compounds, we have used Mycobacterium smegmatis as a lab model for Mycobacterium tuberculosis, the causative agent of TB. M. smegmatis has two genes that encode RNase HI enzymes, rnhA and rnhC, enabling a knockout strategy to investigate the cellular effects of reducing the amount of functional RNase HI in the cell. We show that the rnhC knockout strain of M. smegmatis accumulates R-loops, has an activated SOS response and is sensitised to the antibiotics rifampicin, an inhibitor of RNA polymerase, and suramin, an inhibitor of the SOS response regulator RecA. These observations raise the possibility of RNase HI inhibitors being potential candidates for new antibiotics against M. tuberculosis and other pathogenic bacteria.

 

Related Projects