Presenter: Antoine van Oijen, School of Chemistry, University of Wollongong
Date: 21 April 2016
Time 12.00pm – 1.00pm
Location: Level 3 Seminar Room, 15 Innovation Walk, Monash University, Clayton Campus
Our group is interested in combining fluorescence imaging approaches with molecular manipulation techniques to study biological processes at the single-molecule level. By recording ‘molecular movies’ of individual enzymes we hope to gain new insight into reaction dynamics and mechanisms. In a biological context, most of these enzymes function in concert with other enzymes in multi-protein complexes, so an important direction is the utilization of single-molecule techniques to unravel the orchestration of large macromolecular assemblies. I will discuss recent results of single-molecule studies of the protein machinery responsible for DNA replication. We visualize the dynamic behavior of replication complexes during replication and unravel mechanistic working principles that challenge aspects of the existing textbook view of replication.
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